N-glycan Release Internal QC
$600.00
Due to a lack of tools, the effectiveness of an N-glycan release has been frequently ignored. We have developed a new approach to quantify the effectiveness of N-glycan release across various mammalian samples. Our standards, tentatively named N-glycan Release Internal QC, are added immediately before digestion. Their unique construction enables the release efficiency to be directly determined by downstream data analysis of the final sample data.
Description
How do you assess PNGase-F reaction completeness?
Our Release Internal QC standard was developed with a few key parameters:
- No interference between the release QC glycans and mammalian glycan samples
- Compatible with popular glycan release methods including PNGase-F
- Liberates easily from glycoconjugate, enabling insights especially for incomplete digestions
Applications
Anywhere the glycoprotein amount varies within a batch, or depends upon complete release confirmation, are the best use cases. Here are a few examples:
- Plasma/serum population studies
- Cancer tissue
- Multi-organ batch comparisons
Mix, release, and evaluate.
No changes to your protocol are required, add it to your starting sample and follow your standard workflows.
What’s included?
- One tubes of dried N-glycosylated glycoconjugate.
- Each tube can be used more than 100 times as an internal standard
- QC glycan masses, Skyline document targeting those glycans
Dried samples are stable sealed at room temperature for six months. Record the resuspension and injection volumes, and begin with a low loading to establish an appropriate amount for your system.
